Recombinant DNA (GATE - BT)
Description: GATE NET SLET | |
Number of Questions: 15 | |
Created by: Darshan Khurana | |
Tags: GATE NET SLET Recombinant DNA Technology |
Which of the following is a pair of homologous recombination methods?
In reciprocal recombination,
All of the following are examples of non-homologous recombination methods, except
Which of the following statements are false?
- Transposition occurs by non-replicative mechanism only.
- Homologous recombination between multiple copies of a transposon causes rearrangement of host DNA.
- Homologous recombination between the repeats of transposons may lead to precise or imprecise excision.
- Imprecise excision describes the removal of a transposon plus one of the duplicated target sequences from the chromosome.
Which of the following types of random mutations involve substitution of a purine nucleotide by a pyrimidine?
Which of the following blotting methods is used to check the presence of protein post translational modification?
Assertion (A): Transposition starts by forming a strand transfer complex in which the transposon is connected to the target site through one strand at each end. Reason (R): Replicative transposition proceeds through a cointegrate.
Directions: In the following question, there are two statements, Assertion (A) and Reason (R). Consider both the statements independently and mark your answer as follows:
1. A and R both are correct and R is the correct explanation of A.
2. A and R both are correct and R is not the correct explanation of A.
3. A is correct and R is incorrect.
4. A is incorrect and R is correct.
5. A and R both are incorrect.
Retroviruses are used for inserting a normal gene into the genome to replace an abnormal disease-causing gene in gene therapy of
- ADA deficiency
- Alpha-1-antitrypsin deficiency
- Cystic fibrosis
- Haemophilia b
- Rheumatoid arthritis
Which of the following are examples of random mutagenesis strategies?
P. DOGS Q. StEP R. SeSAM S. Error-prone PCR
Immunoblot is another name of
Which of the following is a method of gene delivery using nanomaterials?
Which of the following methods is/are named after its/their inventor(s)?
- Southern blot
- Northern blot
- Western blot
Match column – I (blotting technique) with column – II (used in detection of) and select the correct option.
Column – I | Column – II |
p. Southern blot | i. Proteins |
q. Northern blot | ii. DNA |
r. Western blot | iii. RNA |
Match column – I (Disease) with column – II (Target tissues for gene therapy) and select the correct option.
Column – I | Column – II |
A. Haemophilia b | 1. Synovial membrane |
B. Cystic fibrosis | 2. Respiratory epithelium |
C. Rheumatoid arthritis | 3. Skin fibroblasts |
Assertion: Gene recombination refers to the exchange of blocks of genetic material among two or more DNA strands. Reason: In site-specific recombination, specialized nucleotide sequences exhibiting some degree of target site specificity are moved between homologous sites within a genome.
Directions: In the following question, there are two statements, Assertion (A) and Reason (R). Consider both the statements independently and mark your answer as follows:
1. A and R both are correct and R is the correct explanation of A.
2. A and R both are correct and R is not the correct explanation of A.
3. A is correct and R is incorrect.
4. A is incorrect and R is correct.
5. A and R both are incorrect.