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Recombinant DNA (GATE)

Description: GATE
Number of Questions: 15
Created by:
Tags: GATE Restriction and Modification Enzymes Biochemistry of Major Metabolic Pathways and Products Bacteriophage and other Viral Vectors Recombinant DNA Technology DNA Replication and Control Recombination and Chromosome Mapping
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Which of the following statements is/are correct?

  1. Endogenous antigens are present on the cell surface along with MHC-I.
  2. The RNA primer synthesised during the replication process in bacteria is removed by DNA polymerase II.
  3. Type III restriction endonucleases cut DNA at defined positions close to or within their recognition sequences.

  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3

Show answer
Correct Option: C
Explanation:

This option is correct because type III restriction endonucleases cut DNA outside of their recognition sequences and require two such sequences in opposite orientations within the same DNA molecule to accomplish cleavage.

Which of the following pairs is not correct?

  1. Atrazine : Photosynthetic inhibitors at photosystem II site B

  2. Glyphosate : Inhibitor of EPSP synthesis

  3. Paraquat : Cell membrane disruptor

  4. Metolachlor : Inhibitor of cell growth and division

  5. Trifluralin : Microtubule assembly inhibitor

Show answer
Correct Option: A
Explanation:

Photosynthetic inhibitors at photosystem II site A type herbicides are chemicals that disrupt photosynthesis, so carbohydrates cannot be produced and the plant dies. These chemicals have activity when applied to leaf tissue, but they are typically used as pre-emergent applications as they are taken up by the roots of newly emerging weeds. For example, Atrazine, Hexazinone, Terbacil, Simazine.

Which of the following pairs is incorrect?

  1. Biopiracy : Exploitation of bioresources without authentic permission

  2. Bioethics : Moral guidance to the problems in biology

  3. Biopotency : Measure of the ability of a material to have a specified biochemical function

  4. Cryopreservation : Preserving germplasm in frozen state

  5. Biolistics : Technique of using very small metal particles coated with desired gene in gene transfer

Show answer
Correct Option: B
Explanation:

This option is correct because bioethics is a set of standards used to regulate own or community activity in relation to biological world.

Directions: Read the given statements and choose the correct option.

Assertion: Non-competitive inhibitors do not compete with the substrate for the enzyme’s active site. Reason: Sulfanilamide is an example of non-competitive inhibitors.

  1. A and R are both correct and R is the correct explanation of A.

  2. A and R are both correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R are both incorrect.

Show answer
Correct Option: C
Explanation:

This option is correct because non-competitive inhibitors do not compete with the substrate for the enzyme’s active site. Instead, they interact with another part of the enzyme and sulfanilamide is an example of competitive inhibitors.

Which of the following statements are false?

  1. Heat-inactivation of serum is done to inactivate prions.
  2. Nude mice refers to transgenic mice.
  3. A humanised antibody is one in which the CDRs are from mouse and the rest is from humans.
  4. B-lymphocytes originate from the bone marrow.

  1. 1 and 2

  2. 1 and 3

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4

Show answer
Correct Option: A
Explanation:

This option is correct.

Which of the following statements is incorrect?

  1. Southern blotting technique depends on similarities between the sequences of probe RNA and experimental RNA.

  2. Electroporation is used to transfer polar molecules like DNA into host cells.

  3. Northern blotting is a laboratory method used to detect specific RNA molecules from a mixture of RNA molecules.

  4. Gel electrophoresis is a basic technique used to separate DNA, RNA or proteins.

  5. PCR is a laboratory technique for amplifying a specific DNA sequence.

Show answer
Correct Option: A
Explanation:

This option is correct because southern blotting technique is a laboratory method used to detect specific DNA molecules among other DNA molecules.  

Which of the following statements is false?

  1. The term enzyme was proposed by Kuhne.

  2. The first enzyme molecule to be isolated in pure crystalline form was urease.

  3. Synthesis of enzymes is regulated by inducer and repressor genes.

  4. DNA ligase was first isolated from bacteriophages.

  5. Ribozyme is an RNA with enzyme activity.

Show answer
Correct Option: C
Explanation:

This option is correct because synthesis of enzymes is regulated by operator and regulator genes.

Match the following:

 
Group I (enzyme class) Group II (example)
A. Oxidoreductase 1. Acetate kinase
B. Transferase 2. Lactate dehydrogenase
C. Hydrolase 3. Oxalate decarboxylase
D. Lyase 4. Sucrase

  1. A - 1, B - 4, C - 3, D - 2

  2. A - 2, B - 1, C - 4, D - 3

  3. A - 3, B - 2, C - 1, D - 4

  4. A - 4, B - 1, C - 2, D - 3

  5. A - 3, B - 4, C - 2, D - 1

Show answer
Correct Option: B
Explanation:

This option is correct because all the class enzymes are correctly matched with their examples.

Lactate dehydrogenase is an example of oxidoreductases. Acetate kinase is an example of transferases. Sucrase is an example of hydrolases. Oxalate decarboxylase is an example of lyases.

Match the following:

 
Column – I (product) Column – II (application)
A. Cyclophosphamide 1. Muscle relaxant
B. Digoxin 2. Anti-cancer agent
C. Lactitol 3. Cardiovascular disorder
D. Pancuronium 4. Sweetener

  1. A - 2, B - 1, C - 3, D - 4

  2. A - 2, B - 1, C - 4, D - 3

  3. A - 2, B - 3, C - 4, D - 1

  4. A - 3, B - 2, C - 4, D - 1

  5. A - 1, B - 2, C - 3, D - 4

Show answer
Correct Option: C
Explanation:

This option is correct because all are correctly matched.  

Directions: Read the given statements and choose the correct option.

Assertion: Flavr Savr tomato was the first commercially grown genetically engineered food to be granted a license for human consumption. Reason: Flavr Savr tomatoes were developed using recombinant DNA techniques to express the trait of early softening of tomato fruit.

  1. A and R are both correct and R is the correct explanation of A.

  2. A and R are both correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R are both incorrect.

Show answer
Correct Option: C
Explanation:

This option is correct.

Which of the following statements is not correct?

  1. Plasmids are circular pieces of double-stranded DNA that exist separately from chromosomal DNA.

  2. Plasmids that can be propagated in cells of two or more different species are called expression vectors.

  3. Shuttle vectors can be used in both eukaryotes and prokaryotes.

  4. A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted for cloning purposes.

  5. Replacement vector is a cloning vector in which part of the normal genome is replaced by foreign DNA.

Show answer
Correct Option: B
Explanation:

This option is correct because plasmids that can be propagated in cells of two or more different species are called shuttle vectors.

Match the following:

 
Column – I Column – II
P. Erythropoietin 1. Lymphoblastic leukaemia
Q. Factor VIII 2. Haemophilia
R. Pegaspargase 3. Anaemia
S. Superoxide dismutase 4. Heart attack

  1. P - 1, Q - 2, R - 3, S - 4

  2. P - 2, Q - 1, R - 4, S - 3

  3. P - 3, Q - 2, R - 1, S - 4

  4. P - 4, Q - 3, R - 2, S - 1

  5. P - 1, Q - 2, R - 4, S - 3

Show answer
Correct Option: C
Explanation:

This option is correct because all are correctly matched in this option.

Which of the following matchings is not correct?

  1. Recombinant DNA technology - combining genes from different species

  2. Restriction fragments - pieces of DNA that have been cut into different lengths

  3. Genomic library - all the genes contained in one kind of organism

  4. Restriction enzymes – DNA-cutting enzymes

  5. DNA fingerprinting - technique for identification of patterns that occur in DNA

Show answer
Correct Option: C
Explanation:

This option is correct because a genomic library is a collection of cloned DNA pieces from a genome.

Match the following:

 
Group I Group II
A. Pyridoxal phosphate 1. D-amino acid oxidase
B. NAD+ 2. Protein kinase A
C. cAMP 3. Serine dehydratase
D. FAD 4. Alcohol dehydrogenases

  1. A - 1, B - 2, C - 3, D - 4

  2. A - 2, B - 1, C - 4, D - 3

  3. A - 2, B - 1, C - 3, D - 4

  4. A - 3, B - 4, C - 2, D - 1

  5. A - 3, B - 2, C - 4, D - 1

Show answer
Correct Option: D
Explanation:

This option is correct.

Directions: Read the given statements and choose the correct option.

Assertion: Zinc-finger nucleases (ZFNs) are artificial restriction enzymes. Reason: The DNA-binding domains of individual ZFNs typically contain between nine and eighteen individual zinc finger repeats.

  1. A and R are both correct and R is the correct explanation of A.

  2. A and R are both correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R are both incorrect.

Show answer
Correct Option: C
Explanation:

This option is correct.

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